HELMZYM (Enzymes)

HELM Quadrazyme

Main enzyme activities:
Endo 1,4-β-D-xylanase
Endo 1,3(4)- β-D-glucanase

Qualitative and quantitative composition of the additive:
The two main components of HELM Quadrazyme are xylanase and β-glucanase.

Xylanase:

endo 1,4-D-xylanase
JUB: 3.2.1.8
CAS: 9025-57-4
EINECS: 232-800-2
Minimum guaranteed activity:

28 000 Unit/g –method I.E.E.B
90 000 Unit/g – method Amano

Substrate: cereal xylans. The enzyme, type endoenzyme, hydrolyses the 1,4,-d-xylosidic links.

β-glucanase:

endo 1,3(4)-β-D-glucanase
JUB: 3.2.1.6
CAS: 62 213- 14-3
EINECS: 263-462-4

Minimum guaranteed activity:

140 000 Units/g –method I.E.E.B
1 500 Units/g – method Amano

Substrate: cereal β-glucans. The enzyme hydrolyses links β-1-3 and links β-1,4-glucosides in the β-glucans.

Physical state, particle size:
HELM Quadrazyme is a slightly yellowish powder, soluble in water, insoluble in ethanol.
Particle sizes: more than 90% through 0,05-0,16 mm.

Manufacturing process:
HELM Quadrazyme is manufactured according to a fermentation process from a selected strain of Aspergillus niger C-25.

Usage

Both enzymes are used as a technological additive. Its action must be associated to target raw materials rich in N.S.P namely cereals such as wheat and barley. It counteracts the antinutritive properties of -glucans and pentosans. This problem can be solved by addition of HELM Quadrazyme.

Efficacy studies have shown that its incorporation into poultry feed improved the metabolisable energy and growth performances of target raw materials: wheat and barley.

We recommend the use of HELM Quadrazyme in poultry feed containing a minimum level of 20% target raw products (wheat/barley).

Minimum dose of HELM Quadrazyme in poultry would then be 15 ppm for feed containing wheat, and 20 ppm for feed containing barley.

For practical use we recommend a premix which is used with 150-250 g/mt.

Other concentrations are available on request.

Advantages of NSP-degrading enzymes in animal nutrition

Cereals (% of composition)

 
barley
oat
rye
triticale
wheat
Enzyme addition - + - + - + - + - +
Chick feed 10 40 20 w.l. 5 20 20 w.l. 30 w.l.
Layer feed (young) 30 w.l. 30 w.l. 15 30 30 w.l. 40 w.l.
Layer feed 40 w.l. 20 40. 20 40 30 w.l. 40 w.l.
Broiler feed 10 40 20 w.l. 5 20 20 40 20 w.l.

w.l. = without limitation

Recommendation for cereal components in compound feed with and without enzymes.

Usage of single enzymes Xylanase and Glucanase
in solid and liquid form

To make the use of enzymes more flexible i.e. only wheat-mixtures or barley-mixtures we are able to offer xylanase and glucanase as single enzymes as well.

Glucanase:

endo 1,3(4) β-glucanase
EG Nr. 19
EC 3.2.1.6

Preparation from Endo 1,3(4) β-glucanase from Aspergillus niger with minimum activity of:
Powder: 1500 AGL/g
Liquid: 200 AGL/g

Xylanase:

endo 1,4-β-xylanase
EG Nr. 21
EC 3.2.1.8

Preparation from Endo 1,4 beta xylanase from Trichoderma longibrachiatum with minimum activity of:
Powder: 1500 AXC/g
Liquid: 200 AXC/g

Both enzymes types we can offer in powder and liquid form for post-pelletizing systems to avoid loss of activity during heat treatment.

Please contact us regarding mixing recommendations and different concentrations depending on your equipment.

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